KMID : 1189220070340040275
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Korean Journal of Reproductive Medicine 2007 Volume.34 No. 4 p.275 ~ p.283
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Expression of Endometriosis Related Genes in the Shed Endometrial Tissues from Menstrual Blood
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Park Chan-Woo
Jeon Jin-Hyun Koong Mi-Kyoung Song In-Ok
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Abstract
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Objective: This study was performed to investigate the expressions of endometriosis related genes in shed endometrial tissues from menstrual blood of patients with or without endometriosis.
Methods: The shed endometrial tissues were collected on 2nd or 3rd day of menstrual cycle with Wallace catheter in patients with endometriosis (n=16) and without endometriosis (n=26). The mRNA expressions of twelve kinds of endometriosis related genes were compared between two groups using semi-quantitative RT-PCR.
Results: The collected shed endometrium was confirmed by histological observation. Expressions of telomerase, c-kit and aromatase mRNA were not detected by RT-PCR in shed endometrial tissues. The mRNA expressions of apoptosis related genes (fas, fas ligand, bcl-2, bax), stem cell factor, estrogen receptor-¥á/¥á, endometriosis protein-I and secretory leukocyte protease inhibitor gene were similar between shed endometrial tissues with endometriosis and without endometriosis.
Conclusion: We could not find the difference of mRNA expressions of tested endometriosis related genes between shed endometrial tissues with or without endometriosis by semi-quantitative RT-PCR analysis. It may be related to the dynamical changes of gene expressions in the endometrium with menstrual cycle.
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KEYWORD
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Endometriosis, Shed endometrial tissues, mRNA expression, Semi-quantitative RT-PCR
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